secDF [2019-01-04 15:05:13]

preprotein translocase subunit (ATPase)

locus: BSU27650

pI: 9.52

mw: 81.47 kDa

protein length: 737 aa

gene length: 2211 bp

function: protein secretion

product: preprotein translocase subunit (ATPase)

essential: no

synonyms: secF,secD

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,826,900 → 2,829,113

The protein

Structure

• [PDB|5XAN] (32% identity)

[SW|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|F21A75744FA0B25D5A251CB57E7A7DC6ABFF1DC7|comN]-[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]
  description: [pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

Biological materials

Mutant

• MGNA-B519 (yrvB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1518 NBRP B. subtilis, Japan]
• SM-GN3 (''secDF-spc''), available in [SW|Anne Galinier]'s and [SW|Boris Görke]'s labs
• BKE27650 (Δ[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATATCCTCCCTT, downstream forward: _UP4_TAAAAAATATCAGGCTGTCC
• BKK27650 (Δ[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATATCCTCCCTT, downstream forward: _UP4_TAAAAAATATCAGGCTGTCC

References

Reviews

• 25212246

Original publications

• 18763711,15995216,18179421